Anti-Mouse PD-L2 (B7-DC) – Purified

Pricing & Details

Product No.C2142
Clone
TY25
Protein
PD-L2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD273
Isotype
Rat
IgG2a κ
Applications
FC
,
IHC FF
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2142-500 µg
500 µg
$105.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified transfected mouse B7-DC cell line
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this CD273 antibody clone TY25 for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB
Other Applications Reported In Literature ?
IHC FF
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone TY25 recognizes an epitope on mouse PD-L2. TY25 does not cross-react with PD-L1.
Antigen Distribution
PD-L2 is expressed on macrophages and a subset of dendritic cells (DCs).
Background
PD-L2 antibody, clone TY25, recognizes mouse programmed death ligand 2 (PDL-2), also known as B7-DC and CD273. PD-L2 is a 42 kDa type I transmembrane glycoprotein that belongs to the B7 family of the immunoglobulin (Ig) receptor superfamily. PD-L2 is an inhibitory ligand primarily expressed on antigen-presenting cells, including macrophages and a subset of dendritic cells (DCs). Its receptor, PD-1, is expressed by CD4-CD8- thymocytes as well as CD4 and CD8 T cells, monocytes, DCs, and B cells upon activation1. Binding of PD-L2 to PD-1 on CD4 and CD8 T cells inhibits T cell receptor (TCR) signaling, negatively regulating T cell proliferation, cytokine production, and cytotoxic activity2. PD-L2 is expressed on many tumor types3,4 and is associated with unfavorable prognosis in patients with solid tumors5.

Antigen Details

Protein
Ligand/Receptor
PD-1, uncharacterized receptor
PubMed
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
.
Immunology

References & Citations

1. Francisco LM, et al. (2010) Immunol Rev. 236:219-242
2. Latchman Y, et al. (2001) Nat Immunol. 2(3):261-8
3. Ahmad SM, et al. (2018) Oncoimmunology. 7:e1390641
4. Panjwani PK, et al. (2018) Hum Pathol. 71:91–9
5. Yang H, et al. (2019) Front Oncol. 9:47
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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