Anti-Mouse Sperm Protein sp56 Antibody (55102)
Anti-Mouse Sperm Protein sp56 Antibody (55102)
Product No.: 55102
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Clone 5F12 Target Mouse Sperm Protein sp56 Formats AvailableView All Product Type Monoclonal Isotype Mouse IgG1 Applications ELISA , IHC , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse, Hamster Host Species Mouse Immunogen Mixture of native and formaldehyde-zinc fixed mouse sperm purified through a Percoll gradient Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling These antibodies are stable for at least on (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco These antibodies may be used in
Immunohistochemistry on zinc-formaldehyde fixed, paraffin-embedded mouse testes to localize sp56 on mouse sperm and in immunoblots or ELISA to detect sp56. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity These antibodies specifically recognize mouse sperm protein sp56. They will react with native or denatured, non-reduced sp56 but not with denatured, reduced sp56. These antibodies do not cross-react with human sperm. Relative antibody titers for antibody binding to solid phase sp56: 55101 = 66pM, 55102 = 4nM, 55103 = 29nM, 55104 = 70nM Background Mouse sperm recognize and bind to ZP3, one of three glycoproteins in the egg’s zona pellucida. Mouse Sperm protein sp56 has the characteristics expected of the sperm protein responsible for recognition of ZP3. Primary sequence data indicates that sp56 is a member of a superfamily of protein receptors. Expression of sp56 is restricted to mouse spermatids and is a peripheral membrane protein located on plasma membrane overlying the sperm’s acrosome. Native sp56 is expressed as a homomultimer. Research Area Reproductive Biology References & CitationsCheng A. et al. 1994. Sperm-Egg Recognition in the Mouse: Characterization of sp56, A Sperm Protein Having Specific Affinity for ZP3. J Cell Biol 125: 867-878. Bookbinder LH et al. 1995. Tissue- and Species-Specific Expression of sp56, a Mouse Sperm Fertilization Protein. Science 269: 86-89. Technical Protocols   Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
