Anti-Mouse TNFα [Clone TN3-19.12] – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse TNFα [Clone TN3-19.12] – Purified in vivo PLATINUM™ Functional Grade
Product No.: T703
Clone TN3-19.12 Target TNF-α Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TNF-alpha, TNFSF2, Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin Isotype IgG Applications in vivo , IP , N , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Armenian Hamster Recommended Dilution Buffer Immunogen Purified Recombinant Mouse TNF-α Product Concentration 2.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2832119 Applications and Recommended Usage? Quality Tested by Leinco WB Additional Applications Reported In Literature ? IP Additional Reported Applications For Relevant Conjugates ? N For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone TN3-19.12 recognizes mouse TNFα. Background TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Antigen Distribution TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells. Ligand/Receptor TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75) NCBI Gene Bank ID UniProt.org Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone TN3-19.12 is primarily used in in vivo mouse studies to neutralize endogenous TNF-alpha (and to some extent TNF-beta) in order to examine the physiological and pathological roles of tumor necrosis factor during immune responses, inflammation, and disease models. Essential Context and Supporting Details:
Specifics:
Summary Table: TN3-19.12 in Mouse Studies
Conclusion: The clone TN3-19.12 (Mouse TNF-alpha Antibody) has specific storage temperature requirements that depend on the duration and condition of storage. Short-term StorageFor short-term storage after reconstitution, the antibody should be kept at 2 to 8°C under sterile conditions for up to 1 month. This refrigeration temperature maintains antibody stability and activity for immediate experimental use. Long-term StorageFor longer-term storage after reconstitution, the antibody should be stored at -20 to -70°C under sterile conditions for up to 6 months. Some suppliers specifically recommend storage at ? -70°C for extended periods to maximize stability. Initial StorageAs supplied in its sterile packaged form before reconstitution, the antibody maintains stability for 12 months when stored at -20 to -70°C. Additional Storage ConsiderationsWhen storing this antibody, it's important to avoid repeated freeze-thaw cycles, which can compromise antibody integrity. For this reason, functional grade preclinical antibodies like clone TN3-19.12 should be aliquoted into working volumes without dilution before freezing. The antibody should be handled in a sterile environment and may require filtration through a 0.2 ?m filter for certain applications. The storage temperature recommendations ensure that this hamster anti-mouse TNF-alpha monoclonal antibody retains its binding capacity and functional activity for research applications. Antibodies and Proteins Commonly Used with TN3-19.12The TN3-19.12 monoclonal antibody, which targets mouse and rat TNF-? (and cross-reacts with TNF-?/lymphotoxin), is widely used in immunology research. Its applications often involve pairing with other antibodies or proteins, especially in multiplex assays and functional experiments. Related Antibodies
Cell and Protein Preparations
Application-Specific Protocols
Summary Table
Key Literature Points
In summary, TN3-19.12 is most commonly paired with biotinylated or fluorescently labeled secondary antibodies (e.g., Poly5160) and relevant isotype controls in sandwich assays and flow cytometry. Recombinant TNF-? and activated T cell supernatants are frequently used as standards or inhibitory agents in functional studies. The clone TN3-19.12 is a monoclonal antibody that has been widely used in scientific research, particularly for its ability to react with rat and mouse tumor necrosis factor-alpha (TNF-?) proteins. Here are some key findings from its citations in scientific literature:
Overall, the TN3-19.12 antibody is a versatile tool for studying TNF-? and its role in various biological processes, as well as for potential therapeutic interventions in inflammatory diseases. References & Citations1.) El-Harith el-HA et al. (2004) Saudi Med J. 25: 135 2.) Adolf GR et al. (1990) Infec Immun. 58: 3996 3.) Kanneganti et al. (2020) bioRxiv 361048 Journal Link 4.) Fhogartaigh, C.N. et al. (2020) Nat Commun 11, 6385 Journal Link 5.) Karki et al. (2021) Cell. 184:149–168 Journal Link 6.) Karki, R. et al. (2021) Cell 184(1):149–168.e17 Journal Link Technical Protocols  N  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
