Anti-Bovine Osteocalcin [Clone OC4-30]
Anti-Bovine Osteocalcin [Clone OC4-30]
Product No.: 50114
Data
Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval. Chondroma: Human bone tumor. Synovial Sarcoma: human osteosarcoma. - -
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Antibody DetailsProduct DetailsReactivity Species Canine ⋅ Chicken ⋅ Goat ⋅ Human ⋅ Porcine ⋅ Rabbit ⋅ Rat ⋅ Sheep Host Species Mouse Immunogen Bovine osteocalcin Product Concentration Lot Specific Formulation PBS, pH7.4, containing 1.0% bovine serum albumin, lyophlized. State of Matter Liquid Product Preparation Purified by immunoaffinity chromatography Storage and Handling Store stock solution (2. 0 mg/ml) in aliquots at -20°C for 1 year, or at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Sandwich ELISA: 1-10ug/ml with immobilized antigen, pair with catalog no. 50120.
Immunoblotting: 1-10ug/ml, reducing or non-reducing conditions. Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Monoclonal Antibody specific to the γ-carboxylated residue at position 17 of human, bovine, rat, rabbit, pig, dog, goat,
sheep, and chicken osteocalcin. Background Osteocalcin is a noncollagenous, 49 amino acid glutamate-rich polypeptide bone matrix protein with a molecular weight of about 5800Da. Osteoblasts produce osteocalcin and incorporate it into the bone matrix. Osteocalcin is released into the circulation from the matrix during bone resorption and, therefore, is considered a marker of bone turnover rather than a specific marker of bone formation. Antigen DetailsFunction Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. NCBI Gene Bank ID UniProt.org Research Area Hormones/Proteins References & CitationsSoos et al. (1986) Biochem J 235: 199. Brindle et al. (1990) Biochem J 268: 615. Prigent et al. (1990) J Biol Chem 265: 9970. Technical Protocols   |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
