Anti-Procollagen Type I C-Peptide (PIP) Antibody

Anti-Procollagen Type I C-Peptide (PIP) Antibody

Product No.: 42043

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Clone
PC8-7
Target
Procollagen Type I C-Peptide (PIP)
Formats AvailableView All
Product Type
Monoclonal
Isotype
IgG1
Applications
ELISA
,
IHC
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactivity Species
Bovine
Canine
Horse
Human
Host Species
Mouse
Immunogen
Human Procollagen Type I C-Peptide
Formulation
PBS, pH 7.4, 1% BSA, lyophilized.
State of Matter
Lyophilized
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
This product does not contain preservative. The stock solution (2. 0mg/ml) can be stored in aliquots at -20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA: 0.2ug/ml with solid phase antigen. For sandwich ELISA, use 42043 on the solid phase and labeled 42024 for detection of binding

Western Blot: 10ug/ml under non-reducing and non-heating conditions.

Immunohistochemistry: 10ug/ml on cultured cells, frozen and paraffin-embedded tissue sections (Antigen retrieval: Proteinase K treatment).
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Monoclonal Antibody specific to non-denatured human, bovine, canine, and equine Procollagen Type I C-peptide (PIP). They do not react with rat or rabbit Procollagen Type 1 C-peptide.
Background
Collagens (types I, II, III, IV, and V) are synthesized as precursor molecules called procollagens. These contain additional peptide sequences, the “propeptidesâ€, at the amino- and carboxy- terminal ends. Propeptides facilitate the winding of procollagen molecules into a triple-helix conformation within the endoplasmic reticulum. These propeptides are cleaved from the collagen triple helix during its secretion after which the collagens polymerize into extracellular collagen fibrils. Therefore, the amount of free propeptides correlates directly with collagen molecule synthesis. Procollagen Type I C-peptide (PIP) has been extensively referenced in studies that correlated collagen levels and disorders such as bone disease, alcoholic liver disease, liver cirrhosis, and scirrhous (Borrmann type IV) adenocarcinoma of the stomach.

Antigen Details

Research Area
Cell Adhesion

References & Citations

Ang XM et al. Macromolecular Crowding Amplifies Adipogenesis of Human Bone Marrow- Derived Mesenchymal Stem Cellsby Enhancing the Pro-Adipogenic Microenvironment. Tissue Engineering Part A Volume 20: 966, 2014. Western blotting Gorur A. et al. COPII-coated membranes function as transport carriers of intracellular procollagen I. J Cell Biol April 20, 2017, PMID 28428367. Immunofluorescence
Indirect Elisa Protocol
IHC
General Western Blot Protocol

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.