Anti-Procollagen Type I C-Peptide (PIP) Antibody
Anti-Procollagen Type I C-Peptide (PIP) Antibody
Product No.: 42043
- -
- -
Clone PC8-7 Target Procollagen Type I C-Peptide (PIP) Formats AvailableView All Product Type Monoclonal Isotype IgG1 Applications ELISA , IHC , WB |
- -
- -
Antibody DetailsProduct DetailsReactivity Species Bovine ⋅ Canine ⋅ Horse ⋅ Human Host Species Mouse Immunogen Human Procollagen Type I C-Peptide Formulation PBS, pH 7.4, 1% BSA, lyophilized. State of Matter Lyophilized Product Preparation Purified by immunoaffinity chromatography Storage and Handling This product does not contain preservative. The stock solution (2. 0mg/ml) can be stored in aliquots at -20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco ELISA: 0.2ug/ml with solid phase antigen. For sandwich ELISA, use 42043 on the solid phase and labeled 42024 for detection of binding
Western Blot: 10ug/ml under non-reducing and non-heating conditions. Immunohistochemistry: 10ug/ml on cultured cells, frozen and paraffin-embedded tissue sections (Antigen retrieval: Proteinase K treatment). Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Monoclonal Antibody specific to non-denatured human, bovine, canine, and equine
Procollagen Type I C-peptide (PIP). They do not react with rat or rabbit Procollagen Type 1 C-peptide. Background Collagens (types I, II, III, IV, and V) are synthesized as precursor molecules called procollagens. These contain additional peptide sequences, the “propeptidesâ€, at the amino- and carboxy- terminal ends. Propeptides facilitate the winding of procollagen molecules into a triple-helix conformation within the endoplasmic reticulum. These propeptides are cleaved from the collagen triple helix during its secretion after which the collagens polymerize into extracellular collagen fibrils. Therefore, the amount of free propeptides correlates directly with collagen molecule synthesis. Procollagen Type I C-peptide (PIP) has been extensively referenced in studies that correlated collagen levels and disorders such as bone disease, alcoholic liver disease, liver cirrhosis, and scirrhous (Borrmann type IV) adenocarcinoma of the stomach. Antigen DetailsResearch Area Cell Adhesion References & CitationsAng XM et al. Macromolecular Crowding Amplifies Adipogenesis of Human Bone Marrow- Derived Mesenchymal Stem Cellsby Enhancing the Pro-Adipogenic Microenvironment. Tissue Engineering Part A Volume 20: 966, 2014. Western blotting Gorur A. et al. COPII-coated membranes function as transport carriers of intracellular procollagen I. J Cell Biol April 20, 2017, PMID 28428367. Immunofluorescence Technical Protocols |
Formats Available
