Anti-Rat CD4 (Clone OX-38) – Purified in vivo PLATINUM™ Functional Grade

Lymphocytes or thymocyte glycoproteins

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM^TM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

OX-38 activity is directed against rat CD4.

CD4 is a 55 kD single-chain type I transmembrane glycoprotein with four domains and is a member of the Ig superfamily^1,2. CD4 functions primarily as a coreceptor for the MHC class II molecule-peptide complex and initiates the early phase of T-cell activation via its association with the T-cell receptor complex and protein tyrosine kinase Lck³. In addition, CD4 T lymphocytes are essential to the host response against infectious disease⁴ and CD4 T cells mediate neuronal damage in infectious and immune-mediated diseases of the central nervous system⁵. In macrophages and natural killer cells, CD4 plays a role in differentiation/activation, cytokine expression and cell migration³.

OX-38 was generated by immunizing mice with lymphocytes or thymocyte glycoproteins and subsequently fusing mouse spleen cells with the P3-X63/Ag 8 cell line⁶. OX-38 is a depleting monoclonal antibody used to study tolerance to allografts^7,8,9,10,11 , Pneumocystis-host interaction⁴, and alopecia¹² in rat models.

CD4 is expressed on most thymocytes, a subset of T cells, and on monocytes/macrophages.

LCK, PTK2/FAK1, P4HB/PDI, IL16, MHCII alpha and beta chains

1. Lynch GW, Turville S, Carter B, et al. Immunol Cell Biol. 84(2):154-165. 2006.
2. Wittlich M, Thiagarajan P, Koenig BW, et al. Biochim Biophys Acta. 1798(2):122-127. 2010.
3. https://www.uniprot.org/uniprotkb/P05540/entry
4. Thullen TD, Ashbaugh AD, Daly KR, et al. Infect Immun. 71(11):6292-6297. 2003.
5. Brunn A, Utermöhlen O, Carstov M, et al. Am J Pathol. 173(1):93-105. 2008.
6. Jefferies WA, Green JR, Williams AF. J Exp Med. 162(1):117-127. 1985.
7. Flavin T, Shizuru J, Seydel K, et al. J Heart Transplant. 9(5):482-488. 1990.
8. Arima T, Lehmann M, Flye MW. Transplantation. 63(2):284-292. 1997.
9. Motoyama K, Arima T, Lehmann M, et al. Surgery. 122(2):213-219. 1997.
10. Qi Z, Riesbeck K, Ostraat O, et al. Transpl Immunol. 5(3):204-211. 1997.
11. Shizuru JA, Seydel KB, Flavin TF, et al. Transplantation. 50(3):366-373. 1990.
12. McElwee KJ, Spiers EM, Oliver RF. Br J Dermatol. 140(3):432-437. 1999.