Anti-Rat CD4 (Clone OX-38) – Biotin

Lymphocytes or thymocyte glycoproteins

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

OX-38 activity is directed against rat CD4.

CD4 is a 55 kD single-chain type I transmembrane glycoprotein with four domains and is a member of the Ig superfamily^1,2. CD4 functions primarily as a coreceptor for the MHC class II molecule-peptide complex and initiates the early phase of T-cell activation via its association with the T-cell receptor complex and protein tyrosine kinase Lck³. In addition, CD4 T lymphocytes are essential to the host response against infectious disease⁴ and CD4 T cells mediate neuronal damage in infectious and immune-mediated diseases of the central nervous system⁵. In macrophages and natural killer cells, CD4 plays a role in differentiation/activation, cytokine expression and cell migration³.

OX-38 was generated by immunizing mice with lymphocytes or thymocyte glycoproteins and subsequently fusing mouse spleen cells with the P3-X63/Ag 8 cell line⁶. OX-38 is a depleting monoclonal antibody used to study tolerance to allografts^7,8,9,10,11 , Pneumocystis-host interaction⁴, and alopecia¹² in rat models.

CD4 is expressed on most thymocytes, a subset of T cells, and on monocytes/macrophages.

LCK, PTK2/FAK1, P4HB/PDI, IL16, MHCII alpha and beta chains

1. Lynch GW, Turville S, Carter B, et al. Immunol Cell Biol. 84(2):154-165. 2006.
2. Wittlich M, Thiagarajan P, Koenig BW, et al. Biochim Biophys Acta. 1798(2):122-127. 2010.
3. https://www.uniprot.org/uniprotkb/P05540/entry
4. Thullen TD, Ashbaugh AD, Daly KR, et al. Infect Immun. 71(11):6292-6297. 2003.
5. Brunn A, Utermöhlen O, Carstov M, et al. Am J Pathol. 173(1):93-105. 2008.
6. Jefferies WA, Green JR, Williams AF. J Exp Med. 162(1):117-127. 1985.
7. Flavin T, Shizuru J, Seydel K, et al. J Heart Transplant. 9(5):482-488. 1990.
8. Arima T, Lehmann M, Flye MW. Transplantation. 63(2):284-292. 1997.
9. Motoyama K, Arima T, Lehmann M, et al. Surgery. 122(2):213-219. 1997.
10. Qi Z, Riesbeck K, Ostraat O, et al. Transpl Immunol. 5(3):204-211. 1997.
11. Shizuru JA, Seydel KB, Flavin TF, et al. Transplantation. 50(3):366-373. 1990.
12. McElwee KJ, Spiers EM, Oliver RF. Br J Dermatol. 140(3):432-437. 1999.