Anti-Rat CNTF (Clone 34738)

Anti-Rat CNTF (Clone 34738)

Product No.: C1440

[product_table name="All Top" skus="C1440"]

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Clone
34738
Target
CNTF
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
HCNTF, Ciliary Neurotrophic Factor
Isotype
IgG2b
Applications
ELISA Cap
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N

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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Mouse
Immunogen
Purified Recombinant Rat CNTF (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:C397 at a concentration of approximately 150-300 ng/ml. A suggested standard for this assay is PN:C329.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Rat CNTF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat CNTF (at 200 ng/ml) is 1 - 3 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Rat Ciliary Neurotrophic Factor (CNTF) (Clone 34738) recognizes an epitope on Rat CNTF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Ciliary neurotrophic factor (CNTF) is a cytokine1 that supports survival and/or differentiation of a variety of neuronal cell types including sensory, sympathetic, and motoneurons.2 With these abilities, CNTF has implications for Parkinson's disease and cell-replacement therapies for other disorders.3 CNTF mimics the biological actions of leptin while overcoming "leptin resistance" and appears to reduce inflammatory signaling cascades associated with lipid accumulation in liver and skeletal muscle. The advantages and disadvantages of CNTF has shown promise as a therapeutic strategy to alleviate obesity-associated diseases.4

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Boschero, AC. et al. (2009) Cytokine 46(1):65-71
2. Thoenen, H. et al. (1994) J Neurobiol. 25: 1436
3. Hagg, T. et al. (2008) J Neurosci. 28: 2231
4. Febbraio, MA. et al. (2008) J Mol Med. 86: 353
Elisa Sandwich Protocol
N

Formats Available

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Prod No.
Description
C1440
Products are for research use only. Not for use in diagnostic or therapeutic procedures.