Anti-Human CNTF

Product No.: C834

[product_table name="All Top" skus="C834"]

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Target
CNTF
Product Type
Polyclonal Antibody
Alternate Names
Ciliary Neurotrophic Factor, HCNTF
Applications
N
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
E. coli-Derived Recombinant Human CNTF
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human CNTF this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for rhCNTF is approximately 5 ng/lane and 20 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human CNTF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CNTF (at 400 ng/ml) is 3 - 8 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human Ciliary Neurotrophic Factor (CNTF) recognizes Human CNTF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Ciliary neurotrophic factor (CNTF) is a cytokine1 that supports survival and/or differentiation of a variety of neuronal cell types including sensory, sympathetic, and motoneurons.2 With these abilities, CNTF has implications for Parkinson's disease and cell-replacement therapies for other disorders.3 CNTF mimics the biological actions of leptin while overcoming "leptin resistance" and appears to reduce inflammatory signaling cascades associated with lipid accumulation in liver and skeletal muscle. The advantages and disadvantages of CNTF has shown promise as a therapeutic strategy to alleviate obesity-associated diseases.4

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Boschero, AC. et al. (2009) Cytokine. 46(1):65-71.
2. Thoenen, H. et al. (1994) J Neurobiol. 25: 1436
3. Hagg, T. et al. (2008) J Neurosci. 28: 2231
4. Febbraio, MA. et al. (2008) J Mol Med. 86: 353
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General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.