Data
![RPA32 antibody [12F3.3] detects RPA32 protein at DNA damage foci by immunofluorescent analysis. Sample: 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody [12F3.3] (56216) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-rpa32-antibody-56216-1.jpg)
RPA32 antibody [12F3.3] detects RPA32 protein at DNA damage foci by immunofluorescent analysis.
Sample: 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RPA32 protein stained by RPA32 antibody [12F3.3] (56216) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Detection of human RPA32 protein using RPA32 12F3.3 monoclonal antibody (56216) in HeLa (IR treated) whole cell extract. No radiation, 1hr, 2hr, and 4hr exposure corresponding to lanes 1 through 4 respectively.
RPA32 antibody detects RPA32 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and blotted with RPA32 antibody (56216) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. - -
- -
Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse Host Species Mouse Immunogen N-terminally 6x-His tagged fusion protein corresponding to full-length human RPA32 expressed in E. coli. Product Concentration Lot Specific Formulation PBS, pH 7.4 State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting,
Immunoprecipitation: use at 0.2-1 ug/mL. Positive control: HeLa, Raji, and 3T3 cells and recombinant protein. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human and mouse RPA32. RPA (Replication Protein A) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32, and 14 kD. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination, and nucleotide excision repair. RPA32 is phosphorylated during the S-phase of the cell cycle, in response to DNA damage, and during apoptosis of Jurkat T-lymphocytes. Function As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. {PubMed:15205463, PubMed:17765923, PubMed:17959650, PubMed:19116208, PubMed:20154705, PubMed:21504906, PubMed:2406247, PubMed:24332808, PubMed:7697716, PubMed:7700386, PubMed:8702565, PubMed:9430682, PubMed:9765279}. NCBI Gene Bank ID UniProt.org Research Area Cancer Research References & Citations1. Iftode, C et al. 1999. Crit Rev Biochem Mol Biol 34: 141-180 2. Bochkarev, A et al. 1999. EMBO 18: 4498-4504 3. Dong, J et al. 1999. Biochem J 337: 311-317 4. Sibenaller, ZA et al. 1998. Biochemistry 37: 12496-12506 5. Stigger, E et al. 1998. J Biol Chem 273: 9337-9343 6. Bochkareva, E et al. 1998. J Biol Chem 273: 3932-3936. Technical ProtocolsICC IF   Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
