Data
![HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMRT antibody [1542] (56217) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-smrt-antibody-56217-1.jpg)
HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMRT antibody [1542] (56217) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. - -
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen A KLH-conjugated synthetic peptide derived from human SMRT corresponding to aa 994-1005 Product Concentration Lot Specific Formulation 10 mM PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by immunoaffinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting and
Immunoprecipitation: use at 1-5 ug/mL. Positive control: HBL100 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human SMRT. Background SMRT (Silencing Mediator for Retinoid and Thyroid receptors) is a 168 kD nuclear protein. Nuclear hormone receptors are transcription factors regulated by ligand binding. In the absence of ligand, there is a physical interaction between the nuclear receptors that do not interact with Hsp90 and a family of corepressors which includes SMRT. SMRT helps target histone deacetylases and other components of a multiprotein repressor complex to the specific target promoter to silence gene transcription. Function Transcriptional corepressor (PubMed:20812024). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 4 have different affinities for different nuclear receptors. Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). {PubMed:18212045, PubMed:20812024, PubMed:23911289}. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & Citations1. Iftode, C et al. 1999. Crit Rev Biochem Mol Biol 34: 141-180 2. Bochkarev, A et al. 1999. EMBO 18: 4498-4504 3. Dong, J et al. 1999. Biochem J 337: 311-317 4. Sibenaller, ZA et al. 1998. Biochemistry 37: 12496-12506 5. Stigger, E et al. 1998. J Biol Chem 273: 9337-9343 6. Bochkareva, E et al. 1998. J Biol Chem 273: 3932-3936. Technical ProtocolsICC IF   Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
