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Immunohistochemistry analysis using Rabbit Anti-SOD2 Polyclonal Antibody (13003). Tissue: muscle fibres. Species: Rat. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (13003) at 1:100. Left: Untreated, Right: treated with 3mmol*kg-1 NAC. Courtesy of: E. Barreiro, IMIM, Spain.
Western blot analysis of Mouse Brain, Rat Brain, and Rat Brain Membrane showing detection of 24.7kDa SOD (Mn) protein using Rabbit Anti-SOD (Mn) Polyclonal Antibody (13003). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 µg). Lane 3: Rat Brain (15 µg). Lane 4: Rat Brain Membrane (15 µg). Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody (13003) at 1:1000 for O/N at 4C. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 24.7kDa. - -
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Antibody DetailsProduct DetailsReactivity Species Bovine ⋅ Canine ⋅ Chicken ⋅ Drosophila ⋅ Guinea Pig ⋅ Hamster ⋅ Human ⋅ Monkey ⋅ Mouse ⋅ Porcine ⋅ Rabbit ⋅ Rat ⋅ Sheep ⋅ Xenopus Host Species Rabbit Immunogen Human Mn SOD Product Concentration 1.0 mg/ml Formulation PBS, pH 7.0, 50% glycerol, and 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by antigen immunoaffinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 0.2-0.5ug/mL.
Positive control: rat brain tissue extract Immunohistochemistry: 1-10ug/mL These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody detects a 25kDa protein on SDS-PAGE immunoblots corresponding to the molecular mass of Mn SOD. This antibody recognizes human, monkey, mouse, rat, bovine, canine, porcine, hamster, guinea pig, rabbit, sheep, chicken, Drosophila, and Xenopus Mn SOD. Background Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme that catalyzes the dismutation of the superoxide radical O2- to oxygen and hydrogen peroxide which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. SODs play an important role in antioxidant defense mechanisms. Three different SOD isoenzymes are found in mammalian cells: SOD1, SOD2, and SOD3. Antigen DetailsFunction Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. {PubMed:10334867}. NCBI Gene Bank ID UniProt.org Research Area Enzymes References & CitationsTechnical Protocols    |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
