Data
Immunoblotting: Detection of VSV-g-tagged fusion protein in 200, 100, and 50ng of E. coli lysate with #18874 diluted 1:25,000. - -
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Antibody DetailsProduct DetailsHost Species Goat Immunogen Synthetic peptide YTDIEMNRLGK conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS, pH 7.2, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by peptide affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000- 1:20,000
Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Polyclonal Antibody specific to VSV-G. Background The Vesicular Stomatitis Virus Glycoprotein (VSV-G) epitope tag is represented by the sequence YTDIEMNRLGK. This tag is often included in recombinant proteins to facilitate identification and isolation of those proteins. Antigen DetailsResearch Area Epitope Tag References & CitationsTechnical Protocols ICC   |
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