Data
Detection of VSV-G-tagged Protein by western blot. Samples: 200200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-VSV-G antibody 18876 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. - -
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Antibody DetailsProduct DetailsHost Species Rabbit Immunogen Synthetic peptide YTDIEMNRLGK conjugated to KLH. Product Concentration 1.0 mg/ml Formulation Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide State of Matter Liquid Product Preparation Purified by peptide immuno-affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000- 1:20,000.
Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate. ELISA: for detection use at a dilution of 1:1,000-1:30,000 These are recommended dilutions. Endusers should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to VSV-G. Antibody was affinity-purified on the peptide. Background The Vesicular Stomatitis Virus Glycoprotein (VSV-G) epitope tag is represented by the sequence YTDIEMNRLGK. This tag is often included in recombinant proteins to facilitate identification and isolation of those proteins. Antigen DetailsResearch Area Epitope Tag References & CitationsTsurumi A et al. 2011. PLoS Genetics 7: e1002086. Xia F et al. 2008. BloS Biology 6: e128. Technical Protocols ICC   |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
