Mouse IgG2a Isotype Control [Clone C1.18.4-mIgG2a] – Recombinant In vivo Purified No Carrier Protein

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This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

The Mouse IgG2a Isotype Control (Clone C1.18.4) is a critical negative control used to measure and eliminate non-specific background binding in assays such as flow cytometry, immunohistochemistry, and ELISA. Traditionally produced via hybridoma, this classic clone has now been engineered as a recombinant antibody expressed in mammalian HEK293 cells.

The Recombinant Advantage:

While traditional hybridomas are susceptible to genetic drift, loss of antibody expression, or the production of extra endogenous light chains, our recombinant HEK293-expressed C1.18.4 guarantees absolute sequence fidelity. By cloning the exact heavy and light chain sequences from the original hybridoma into a stable HEK293 expression system, this version eliminates the risk of multi-chain impurities. The result is a highly pure, structurally defined isotype control that offers unparalleled lot-to-lot consistency, ensuring that your background measurements remain perfectly reproducible across long-term studies. Furthermore, this version is produced in a serum-free (FBS-free) culture, completely eliminating contaminating bovine IgGs and animal-derived variables to deliver an exceptionally clean background control.