Recombinant Human CCL22/MDC

Recombinant Human CCL22/MDC

Product No.: M171

[product_table name="All Top" skus="M171"]

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Alternate Names
MDC, A-152E5.1, ABCD-1, DC/B-CK, MGC34554, SCYA22, STCP-1
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Human

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Product Size
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Background

Chemokine (C-C motif) ligand 22, also known as CCL22 is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4.1 CCL22 selectively chemo-attract Th2 cytokine-producing cells2,3 and acts as an important activator of eosinophils once these cells have migrated into tissue.4

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human MDC was determined by by its ability to chemoattract human T-lymphoblastoid CEM-NKR cells (Howell, D.N. et al., 1985, J. Immunol. 134:971 - 976) and its ability to chemoattract mouse BaF/3 cells transfected with hCCR4. The expected ED<sub>50</sub> for these effects are typically 3 - 9 ng/ml and 0.5 - 3 ng/ml, respectively.
Protein Accession No.
Amino Acid Sequence
gpyganmeds vccrdyvryr lplrvvkhfy wtsdscprpg vvlltfrdke icadprvpwv kmilnklsq
N-terminal Sequence Analysis
Gly25
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CCL22 is Mr 8 kDa.
Predicted Molecular Mass
8
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Sozzani, S. et al. (2001) Eur. J. Immunol. 31:812
2. Elias, CG. et al. (1998) J Immunol. 161:5027
3. Gray, PW. et al. (1997) J Exp Med. 185:1604
4. Teixeira, MM. et al. (2003) J Leuko Biol. 73:356
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.