Purified Recombinant Mouse MDC (>98%)
<0.1 EU/µg as determined by the LAL method
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse CCL22 this polyclonal antibody can be used at a concentration of 0.1 µg/ml.
Other Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse CCL22/MDC.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Mouse Chemokine (C-C Motif) Ligand 22 (CCL22) recognizes Mouse CCL22. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Chemokine (C-C motif) ligand 22, also known as CCL22 is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4.1 CCL22 selectively chemo-attract Th2 cytokine-producing cells2,3 and acts as an important activator of eosinophils once these cells have migrated into tissue.4
NCBI Gene Bank ID
References & Citations
1. Sozzani, S. et al. (2001) Eur. J. Immunol. 31:812
2. Elias, CG. et al. (1998) J Immunol. 161:5027
3. Gray, PW. et al. (1997) J Exp Med. 185:1604
4. Teixeira, MM. et al. (2003) J Leuko Biol. 73:356
Products are for research use only. Not for use in diagnostic or therapeutic procedures.