Anti-Human CCL22 (Clone 57226)

Pricing & Details

Product No.M1092
Clone
57226
Protein
CCL22
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Chemokine (C-C Motif) Ligand 22, MDC, A-152E5.1, ABCD-1, DC/B-CK, MGC34554, SCYA22, STCP-1
Isotype
Mouse
IgG2b
Applications
ELISA Cap
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
M1092-25 µg
25 µg
$195.00
In stock
Max:
Min: 1
Step: 1
M1092-100 µg
100 µg
$430.00
In stock
Max:
Min: 1
Step: 1
M1092-500 µg
500 µg
$935.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CCL22 (Accession # O00626.1)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
This lyophilized monoclonal antibody should be stored desiccated at -20°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:M129 at a concentration of approximately 50 ng/ml. A suggested standard for this assay is PN:M171.
Western Blotting: To detect Human CCL22 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL22 is 5-50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M171.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human MDC bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MDC (at 10 ng/ml) is 0.6 - 3 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Chemokine (C-C Motif) Ligand 22 (CCL22) (Clone 57226) recognizes an epitope on Human CCL22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Chemokine (C-C motif) ligand 22, also known as CCL22 is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4.1 CCL22 selectively chemo-attract Th2 cytokine-producing cells2,3 and acts as an important activator of eosinophils once these cells have migrated into tissue.4

Antigen Details

Protein
PubMed

References & Citations

1. Sozzani, S. et al. (2001) Eur. J. Immunol. 31:812
2. Elias, CG. et al. (1998) J Immunol. 161:5027
3. Gray, PW. et al. (1997) J Exp Med. 185:1604
4. Teixeira, MM. et al. (2003) J Leuko Biol. 73:356
Elisa Sandwich Protocol
General Western Blot Protocol

Formats Available

Prod No.
Description
M1092
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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