Recombinant Human IL-2

Recombinant Human IL-2

Product No.: I-184

[product_table name="All Top" skus="I-184"]

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Prod. No.I-184
Expression Host
E. coli Cells

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SDS Page
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IL-2 is a 17.2 kD cytokine signaling protein that is the regulator of the proliferative and suppressive phases of the immune response, and stimulates growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. It functions as a primary regulator of T cell homeostasis. Il-2 regulates the activities of white blood cells and plays a role in the immune system via “self” and “non-self” discrimination. IL-2 is a prime candidate in immunotherapeutics for both increasing T cell levels/function and for augmenting vaccine-elicited viral-specific T cell responses. IL-2 signal can be transduced via 3 different signaling pathways that include the JAK-STAT, PI3K/Akt/mTOR and MAPK/ERK pathways. Aldesleukin is a form of recombinant interleukin-2 for the treatment of cancers including malignant melanoma and renal cell cancer. Furthermore, there is additional therapeutic potential for IL-2 mAb use with transplants and autoimmune disease.

Protein Details

>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-2 was determined in a cell proliferation assay using an IL-2 dependent murine cytotoxic T cell line, CTLL-2 (Gearing, A.J.H. and C.B. Bird in Lymphokines and Interferons, a Practical Approach. 1987, M.J. Clemens, A.G. Morris and A.J.H. Gearing, eds., IRL Press, p. 295). The expected ED<sub>50</sub> for this effect is typically 0.25 - 0.5 ng/ml.
Protein Accession No.
Amino Acid Sequence
maptssstkk tqlqlehlll dlqmilngin nyknpkltrm ltfkfympkk atelkhlqcl eeelkpleev lnlaqsknfh lrprdlisni nvivlelkgs ettfmceyad etativefln rwitfcqsii stlt
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-2 is Mr 15 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 13 kDa.
Predicted Molecular Mass
This recombinant protein was lyophilized from 0.2 µm filtered solutin in 30%(v/v) Acetonitrile and 0.1%(v/v) TFA.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Schachter, J. et al. (2009) Cytotherapy 11: 206
2. Sodora, DL. et al. (2009) Curr HIV Res. 7: 83
3. Alcocer-Varela, J. et al. (2009) Autoimmun Rev. 9(1):34-9.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.