Recombinant Human IL-2

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Pricing & Details

Product. No.I-184
Alternate Names
Interleukin-2, TCGF, Lymphokine, T-Cell Growth Factor
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-184-10 µg
10 µg
$199.00
In stock
Max:
Min: 1
Step: 1
I-184-50 µg
50 µg
$625.00
In stock
Max:
Min: 1
Step: 1

Data

Background

IL-2 is a 17.2 kD cytokine signaling protein that is the regulator of the proliferative and suppressive phases of the immune response, and stimulates growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. It functions as a primary regulator of T cell homeostasis. Il-2 regulates the activities of white blood cells and plays a role in the immune system via “self” and “non-self” discrimination. IL-2 is a prime candidate in immunotherapeutics for both increasing T cell levels/function and for augmenting vaccine-elicited viral-specific T cell responses. IL-2 signal can be transduced via 3 different signaling pathways that include the JAK-STAT, PI3K/Akt/mTOR and MAPK/ERK pathways. Aldesleukin is a form of recombinant interleukin-2 for the treatment of cancers including malignant melanoma and renal cell cancer. Furthermore, there is additional therapeutic potential for IL-2 mAb use with transplants and autoimmune disease.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-2 was determined in a cell proliferation assay using an IL-2 dependent murine cytotoxic T cell line, CTLL-2 (Gearing, A.J.H. and C.B. Bird in Lymphokines and Interferons, a Practical Approach. 1987, M.J. Clemens, A.G. Morris and A.J.H. Gearing, eds., IRL Press, p. 295). The expected ED50 for this effect is typically 0.25 - 0.5 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
maptssstkk tqlqlehlll dlqmilngin nyknpkltrm ltfkfympkk atelkhlqcl eeelkpleev lnlaqsknfh lrprdlisni nvivlelkgs ettfmceyad etativefln rwitfcqsii stlt
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-2 is Mr 15 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 13 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and is supplied in a solution of 50 mM sodium acetate (NaOAc), pH 4.0.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Schachter, J. et al. (2009) Cytotherapy 11: 206
2. Sodora, DL. et al. (2009) Curr HIV Res. 7: 83
3. Alcocer-Varela, J. et al. (2009) Autoimmun Rev. 9(1):34-9.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.