Anti-Human IL-2

Pricing & Details

Product No.I-426
Protein
IL-2
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Interleukin-2, TCGF, Lymphokine, T-Cell Growth Factor
Applications
ELISA Indirect
,
IHC FFPE
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-426-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
I-426-100 µg
100 µg
$409.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human IL-2 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as PN:G505. The detection sensitivity of this indirect ELISA for Human IL-2 is approximately 0.2-0.6 ng/well.
Western Blotting: To detect Human IL-2 this polyclonal antibody can be used at a concentration of 0.5 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-184.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human IL-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-2 (at 2.0 ng/ml) is 0.05-0.15 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Interleukin-2 (IL-2) recognizes Human IL-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
IL-2 is a 17.2 kD cytokine signaling protein that is the regulator of the proliferative and suppressive phases of the immune response, and stimulates growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. It functions as a primary regulator of T cell homeostasis. Il-2 regulates the activities of white blood cells and plays a role in the immune system via “self” and “non-self” discrimination. IL-2 is a prime candidate in immunotherapeutics for both increasing T cell levels/function and for augmenting vaccine-elicited viral-specific T cell responses. IL-2 signal can be transduced via 3 different signaling pathways that include the JAK-STAT, PI3K/Akt/mTOR and MAPK/ERK pathways. Aldesleukin is a form of recombinant interleukin-2 for the treatment of cancers including malignant melanoma and renal cell cancer. Furthermore, there is additional therapeutic potential for IL-2 mAb use with transplants and autoimmune disease.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Schachter, J. et al. (2009) Cytotherapy 11: 206
2. Sodora, DL. et al. (2009) Curr HIV Res. 7: 83
3. Alcocer-Varela, J. et al. (2009) Autoimmun Rev. 9(1):34-9.
Indirect Elisa Protocol
IHC
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.