Purified Recombinant Human IL-2 (Accession # NP_000577)
<0.1 EU/µg as determined by the LAL method
This purified antibody is 0.2 µm filtered and lyophilized from phosphate buffered saline (PBS), pH 6.2 with 5% Trehalose with no carrier proteins or preservatives.
Storage and Handling
This lyophilized monoclonal antibody should be stored desiccated at -20 to -70⁰C. After reconstitution, this monoclonal antibody can be stored at 2-8⁰C for one month. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20 to -70⁰C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-2. A suggested method would be to stain cells expressing IL-2 with 0.25 µg per 1-5 x 106 cells in a total staining volume of µl followed by PN:M1189.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human IL-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-2 (at 2 ng/ml) is 0.015 - 0.03 µg/ml.
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Clone L032 recognizes human IL-2.
Interleukin-2 (IL-2) is a potent T-cell mitogenic cytokine1 that functions as a primary regulator of T cell homeostasis and has been considered a prime candidate immunotherapeutics, both for increasing T cell levels/function and for augmenting vaccine-elicited viral-specific T cell responses.2 IL-2 plays a key role in setting the balance between immunity and tolerance. This cytokine has a dual role as the regulator of the two main phases of the immune response (proliferative and suppressive).3 IL-2 also stimulates growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
References & Citations
1. Schachter, J. et al. (2009) Cytotherapy 11: 206
2. Sodora, DL. et al. (2009) Curr HIV Res. 7: 83
3. Alcocer-Varela, J. et al. (2009) Autoimmun Rev. 9(1):34-9.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.