Anti-Human IL-2 (Clone 5334)

Pricing & Details

Product No.I-172
Clone
5334
Protein
IL-2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Interleukin-2, TCGF, Lymphokine, T-Cell Growth Factor
Isotype
Mouse
IgG1
Applications
CyTOF®
,
FC
,
ICC
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-172-25 µg
25 µg
$200.00
In stock
Max:
Min: 1
Step: 1
I-172-100 µg
100 µg
$440.00
In stock
Max:
Min: 1
Step: 1
I-172-500 µg
500 µg
$935.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human IL-2 (Accession # NP_000577)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This purified antibody is 0.2 µm filtered and lyophilized from phosphate buffered saline (PBS), pH 6.2 with 5% Trehalose with no carrier proteins or preservatives.
Storage and Handling
This lyophilized monoclonal antibody should be stored desiccated at -20 to -70⁰C. After reconstitution, this monoclonal antibody can be stored at 2-8⁰C for one month. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20 to -70⁰C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-2. A suggested method would be to stain cells expressing IL-2 with 0.25 µg per 1-5 x 106 cells in a total staining volume of µl followed by PN:M1189.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human IL-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-2 (at 2 ng/ml) is 0.015 - 0.03 µg/ml.
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone L032 recognizes human IL-2.
Background
Interleukin-2 (IL-2) is a potent T-cell mitogenic cytokine1 that functions as a primary regulator of T cell homeostasis and has been considered a prime candidate immunotherapeutics, both for increasing T cell levels/function and for augmenting vaccine-elicited viral-specific T cell responses.2 IL-2 plays a key role in setting the balance between immunity and tolerance. This cytokine has a dual role as the regulator of the two main phases of the immune response (proliferative and suppressive).3 IL-2 also stimulates growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Antigen Details

Protein
PubMed

References & Citations

1. Schachter, J. et al. (2009) Cytotherapy 11: 206
2. Sodora, DL. et al. (2009) Curr HIV Res. 7: 83
3. Alcocer-Varela, J. et al. (2009) Autoimmun Rev. 9(1):34-9.
Flow Cytometry

Formats Available

Prod No.
Description
I-172
Products are for research use only. Not for use in diagnostic or therapeutic procedures.