Anti-Human IL-2 (Clone 5334)

Purified Recombinant Human IL-2 (Accession # NP_000577)

This purified antibody is 0.2 µm filtered and lyophilized from phosphate buffered saline (PBS), pH 6.2 with 5% Trehalose with no carrier proteins or preservatives.

This lyophilized monoclonal antibody should be stored desiccated at -20 to -70⁰C. After reconstitution, this monoclonal antibody can be stored at 2-8⁰C for one month. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20 to -70⁰C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Human IL-2 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-2 (at 2 ng/ml) is 0.015 - 0.03 µg/ml.
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Clone L032 recognizes human IL-2.

Interleukin-2 (IL-2) is a potent T-cell mitogenic cytokine¹ that functions as a primary regulator of T cell homeostasis and has been considered a prime candidate immunotherapeutics, both for increasing T cell levels/function and for augmenting vaccine-elicited viral-specific T cell responses.² IL-2 plays a key role in setting the balance between immunity and tolerance. This cytokine has a dual role as the regulator of the two main phases of the immune response (proliferative and suppressive).³ IL-2 also stimulates growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

1. Schachter, J. et al. (2009) Cytotherapy 11: 206
2. Sodora, DL. et al. (2009) Curr HIV Res. 7: 83
3. Alcocer-Varela, J. et al. (2009) Autoimmun Rev. 9(1):34-9.