Recombinant Rat IL-2

IL-2 is a 17.2 kD cytokine signaling protein that is the regulator of the proliferative and suppressive phases of the immune response, and stimulates growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. It functions as a primary regulator of T cell homeostasis. Il-2 regulates the activities of white blood cells and plays a role in the immune system via “self” and “non-self” discrimination. IL-2 is a prime candidate in immunotherapeutics for both increasing T cell levels/function and for augmenting vaccine-elicited viral-specific T cell responses. IL-2 signal can be transduced via 3 different signaling pathways that include the JAK-STAT, PI3K/Akt/mTOR and MAPK/ERK pathways. Aldesleukin is a form of recombinant interleukin-2 for the treatment of cancers including malignant melanoma and renal cell cancer. Furthermore, there is additional therapeutic potential for IL-2 mAb use with transplants and autoimmune disease.

The biological activity of Rat Interleukin-2 is determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells. The expected ED₅₀ for this effect is 2.5 x 10⁶ units/mg.

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Ala21 & Met

Solution

The predicted molecular weight of Recombinant Rat IL-2 is Mr 16 kDa.

16

This recombinant protein was 0.2 µm filtered and is supplied in a solution of sodium acetate (NaOAc) and Glycerol.

Working aliquots of this recombinant protein solution are stable for up to six months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

Dry Ice

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3. Alcocer-Varela, J. et al. (2009) Autoimmun Rev. 9(1):34-9.