Recombinant Human Leptin

Leptin is a neuroendocrine hormone secreted from adipose tissue that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. Leptin is one of the most important adipose derived hormones.¹ It interacts with six types of receptors and once it binds to OB-Receptor it activates the stat3, which is phosphorylated and travels to the nucleus to effect changes in gene expression. One of the main effects on gene expression is the down-regulation of the expression of endocannabinoids.² High leptin levels are associated with psoriasis.³

The biological activity of Human Leptin was determined by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BaF3 cells. The expected ED₅₀ for this effect is 0.4 - 2 ng/ml.

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Met

Lyophilized

The predicted molecular weight of Recombinant Human Leptin is Mr 16 kDa.

16

This recombinant protein was 0.2 µm filtered and lyophilized from Tris-HCL and NaCL pH 8.5.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Mantzoros, CS. et al.(2006) Nat Clin Pract Endocrinol Metab. 2: 318
2. Hill, RA. et al.(2002) Int. J. Obes. Relat. Metab. Disord. 26: 1407
3. Chen, YJ. et al.(2008) Arch Derm. 144: 1571