Anti-Mouse Leptin – Biotin
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen E. coli-Derived Recombinant Mouse Leptin (Accession # Q544U0) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831103 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse Leptin this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L117. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:L115 as the capture antibody at 0.2-0.8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse Leptin. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse Leptin recognizes Mouse Leptin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Leptin is a neuroendocrine hormone secreted from adipose tissue that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. Leptin is one of the most important adipose derived hormones.1 It interacts with six types of receptors and once it binds to OB-Receptor it activates the stat3, which is phosphorylated and travels to the nucleus to effect changes in gene expression. One of the main effects on gene expression is the down-regulation of the expression of endocannabinoids.2 High leptin levels are associated with psoriasis.3 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Mantzoros, CS et al.(2006) Nat Clin Pract Endocrinol Metab. 2: 318 2. Hill, RA. et al.(2002) Int. J. Obes. Relat. Metab. Disord. 26: 1407 3. Chen, YJ. et al.(2008) Arch Derm. 144: 1571 Technical ProtocolsELISA Det  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
