Anti-Mouse Leptin
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse Leptin (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831105 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/ml. A suitable detection antibody is PN:L111 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:L117. Western Blotting: To detect Mouse Leptin this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Leptin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L117. Additional Applications Reported In Literature ? Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 5-15 µg/ml. Neutralization: This antibody is useful for neutralization of Mouse Leptin bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse Leptin (at 2.5 ng/ml) is 0.05-0.3 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Anti-Mouse Leptin recognizes Mouse Leptin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Leptin is a neuroendocrine hormone secreted from adipose tissue that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. Leptin is one of the most important adipose derived hormones.1 It interacts with six types of receptors and once it binds to OB-Receptor it activates the stat3, which is phosphorylated and travels to the nucleus to effect changes in gene expression. One of the main effects on gene expression is the down-regulation of the expression of endocannabinoids.2 High leptin levels are associated with psoriasis.3 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Mantzoros, CS. et al.(2006) Nat Clin Pract Endocrinol Metab. 2: 318 2. Hill, RA. et al.(2002) Int. J. Obes. Relat. Metab. Disord. 26: 1407 3. Chen, YJ. et al.(2008) Arch Derm. 144: 1571 Technical Protocols IHC FF N  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
