Recombinant Mouse Leptin

Home/Recombinant Proteins/Recombinant Mouse Leptin

Pricing & Details

Product. No.L117
Alternate Names
Obesity Protein (OB), B219, LEP, OBS
Expression Host
E. coli
Species
Mouse
Prod No.
Size
Price
Avail.
Qty
Add to cart
L117-1 mg
1 mg
$229.00
In stock
Max:
Min: 1
Step: 1
L117-5 mg
5 mg
$559.00
In stock
Max:
Min: 1
Step: 1

Background

Leptin is a neuroendocrine hormone secreted from adipose tissue that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. Leptin is one of the most important adipose derived hormones.1 It interacts with six types of receptors and once it binds to OB-Receptor it activates the stat3, which is phosphorylated and travels to the nucleus to effect changes in gene expression. One of the main effects on gene expression is the down-regulation of the expression of endocannabinoids.2 High leptin levels are associated with psoriasis.3

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
Leinco's Mouse Leptin has been shown to be biologically active in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 µg Leptin/gm of body weight for 14 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed compared to saline-treated controls.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
mvpiqkvqdd tktliktivt rindishtqs vsakqrvtgl dfipglhpil slskmdqtla vyqqvltslp sqnvlqiand lenlrdllhl lafskscslp qtsglqkpes ldgvleasly stevvalsrl qgslqdilqq ldvspec
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse Leptin is Mr 16 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Mantzoros, CS. et al.(2006) Nat Clin Pract Endocrinol Metab. 2: 318
2. Hill, RA. et al.(2002) Int. J. Obes. Relat. Metab. Disord. 26: 1407
3. Chen, YJ. et al.(2008) Arch Derm. 144: 1571
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.