Anti-Human Leptin (Clone 44802)

Pricing & Details

Product No.L109
Clone
44802
Protein
Leptin
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Obesity Protein (OB), B219, LEP, OBS
Isotype
Mouse
IgG1
Applications
ELISA Cap
,
N
Prod No.
Size
Price
Avail.
Qty
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L109-25 µg
25 µg
$180.00
In stock
Max:
Min: 1
Step: 1
L109-100 µg
100 µg
$430.00
In stock
Max:
Min: 1
Step: 1
L109-500 µg
500 µg
$935.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human Leptin (Accession # P41159)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human OB bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human OB (at 7.5 ng/ml) is 0.4 - 1.6 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Leptin (Clone 44802) recognizes an epitope on Human Leptin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Leptin is a neuroendocrine hormone secreted from adipose tissue that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. Leptin is one of the most important adipose derived hormones.1 It interacts with six types of receptors and once it binds to OB-Receptor it activates the stat3, which is phosphorylated and travels to the nucleus to effect changes in gene expression. One of the main effects on gene expression is the down-regulation of the expression of endocannabinoids.2 High leptin levels are associated with psoriasis.3

Antigen Details

Protein
PubMed

References & Citations

1. Mantzoros, CS. et al.(2006) Nat Clin Pract Endocrinol Metab. 2: 318
2. Hill, RA. et al.(2002) Int. J. Obes. Relat. Metab. Disord. 26: 1407
3. Chen, YJ. et al.(2008) Arch Derm. 144: 1571
Elisa Sandwich Protocol

Formats Available

Prod No.
Description
L109
Products are for research use only. Not for use in diagnostic or therapeutic procedures.