Recombinant Human TWEAK

TNF-related weak inducer of apoptosis (TWEAK) is a member of the TNF superfamily. This cytokine has overlapping signaling functions with TNF but displays a much wider tissue distribution. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. (1). It has pleiotropic biological functions including proapoptotic, proangiogenic and proinflammatory activities (2-3). TWEAK also induces skeletal muscle atrophy (4). It can function as both as a type II transmembrane protein and as a cleaved soluble protein. TWEAK is the ligand for the FN14/TWEAKR receptor.

The biological activity of Human TNF-Related Weak Inducer of Apoptosis is determined by the dose-dependent stimulation of IL-8 production by Human PBMC. The expected ED₅₀ for this effect is less than 10.0 ng/ml.

Histidine Tag

hhhhhhhrsa pkgrktrarr aiaahyevhp rpgqdgaqag vdgtvsgwee arinsssplr ynrqigefiv traglyylyc qvhfdegkav ylkldllvdg vlalrcleef sataasslgp qlrlcqvsgl lalrpgsslr irtlpwahlk aapfltyfgl fqv

Met

Lyophilized

The predicted molecular weight of Recombinant Human TWEAK is 18.3 kDa.

18.3

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with BSA as a carrier protein. pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

1. Aaron, JW. et al. (2006) Molecular Endo. 20:2528
2. Burkly, LC. et al. (2005) Oncogene 24:2613
3. Young, S. et al. (2003) Biochem. Biophys. Res. Commun. 305:789
4. Kumar, A. et al. (2007) FASEB J. 21:1857