Recombinant Mouse TWEAK

TNF-related weak inducer of apoptosis (TWEAK), also known as TNFSF12, is a member of the TNF superfamily. This cytokine has overlapping signaling functions with TNF but displays a much wider tissue distribution. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. (1). It has pleiotropic biological functions including proapoptotic, proangiogenic and proinflammatory activities (2-3). TWEAK also induces skeletal muscle atrophy (4). It can function as both as a type II transmembrane protein and as a cleaved soluble protein. TWEAK is the ligand for the FN14/TWEAKR receptor.

The biological activity of Mouse TWEAK was determined by a cell proliferation assay using HUVEC hUmbilical vein endothelial cells. The expected ED₅₀=0.03-0.3 ug/ml.

Histidine Tag

mraiaah yevhprpgqd gaqagvdgtv sgweetkins ssplrydrqi geftviragl yylycqvhfd egkavylkld llvngvlalr cleefsataa sspgpqlrlc qvsgllplrp gsslrirtlp wahlkaapfl tyfglfqvhh hhhhh

Met

Lyophilized

The predicted molecular weight of Recombinant Mouse TWEAK is Mr 17 kDa.

17

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Aaron, JW. et al. (2006) Molecular Endo. 20:2528
2. Burkly, LC. et al. (2005) Oncogene 24:2613
3. Young, S. et al. (2003) Biochem. Biophys. Res. Commun. 305:789
4. Kumar, A. et al. (2007) FASEB J. 21:1857