Anti-Human TWEAK – Biotin

Pricing & Details

Product No.T197
Protein
TWEAK
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
TNF-Related Weak Inducer of Apoptosis, TNFSF12, DR3LG, Apo3-Ligand
Applications
ELISA Det
,
ICC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
T197-50 µg
50 µg
$500.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Goat
Host Species
Human
Immunogen
Purified Recombinant Human TWEAK (Accession No.: Q4ACW9)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1 -0.4 µg/ml when used in conjunction with PN:T544 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Goat TWEAK.
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Other Applications Reported In Literature ?
Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Human Anti-Human TNF-Related Weak Inducer of Apoptosis (TWEAK) recognizes Human TWEAK. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
TNF-related weak inducer of apoptosis (TWEAK) is a member of the TNF ligand family1 that has pleiotropic biological functions including proapoptotic, proangiogenic and proinflammatory activities.2 TWEAK induces skeletal muscle atrophy.3 TWEAK functions both as a type II transmembrane protein and as a cleaved soluble protein. TWEAK transcripts are expressed in heart, brain, skeletal muscle, pancreas, immune system, ovary, and placenta.4

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Burkly, LC. et al. (2005) Oncogene 24:2613
2. Young, LS. et al. (2003) Biochem Biophys Res Commun. 305:789
3. Kumar, A. et al. (2007) FASEB J. 21:1857
4. Hsueh, AJW. et al. (2006) Molecular Endo. 20:2528
Indirect Elisa Protocol
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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