Data
BackgroundCXCL5 is an inflammatory chemokine that belongs to the CXC chemokine family1 that has a role in the development of cardiovascular diseases.2 CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils.1 It is expressed by epithelial cells within colorectal mucosa is a chemoattractant for neutrophils and has been implicated in Crohn's disease and ulcerative colitis.3 CXCL5 is important in growth and development of colorectal cancer, implicating a future role in both cancer therapy and diagnosis.4 Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01 EU/µg as determined by the LAL method Biological Activity The biological activity of Mouse LIX was determined by its ability to induce myeloperoxidase release from cytochalasin-B treated human
neutrophils (Schröder, J.M. et al., 1987, J. Immunol. 139:3474), or chemotaxis of Baf/3 hCXCR-2 transfected cells. The expected ED50 for this effect is typically 1 - 3 μg/ml or 0.03 - 0.1 μg/ml, respectively. Expression Host E. coli Protein Accession No. Amino Acid Sequence viaate lrcvcltvtp kinpklianl evipagpqcp tveviaklkn qkevcldpea pvikkiiqki lgsdkkka N-terminal Sequence Analysis Val45 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Mouse CXCL5 is Mr 8 kDa. Formulation This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient PubMed NCBI Gene Bank References & Citations1. Rajashekhar, G. et al. (2007) Physiol Genomics 31: 104 2. Mahsa, M. et al. (2008) Molec Diagnosis & Therapy 12: 391 3. Wågsäter, D. et al. (2007) International J Oncol. 31: 97 4. Nagelkerke, F. et al. (2008) Clinical Cancer Research 14: 2276 IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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