Anti-Human CXCL5 (Clone 33160)
Anti-Human CXCL5 (Clone 33160)
Product No.: C1386
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Clone 33160 Target CXCL5 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names AMCF-II, Epithelial-Derived Neutrophil-Activating Peptide 78 (ENA-78), GCP-2, Scyb5, Scyb6, Lipopolysaccharide-Induced CXC Chemokine (LIX) Isotype IgG1 Applications IHC FFPE , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human CXCL5 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828442 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human CXCL5 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL5 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E109. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 - 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human CXCL5 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CXCL5 (at 30 ng/ml) is 3 - 15 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human CXCL5 (Clone 33160) recognizes an epitope on Human CXCL5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background CXCL5 is an inflammatory chemokine that belongs to the CXC chemokine family1 that has a role in the development of cardiovascular diseases.2 CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils.1 It is expressed by epithelial cells within colorectal mucosa is a chemoattractant for neutrophils and has been implicated in Crohn's disease and ulcerative colitis.3 CXCL5 is important in growth and development of colorectal cancer, implicating a future role in both cancer therapy and diagnosis.4 Antigen DetailsPubMed References & Citations1. Rajashekhar, G. et al. (2007) Physiol Genomics 31: 104 2. Mahsa, M. et al. (2008) Molec Diagnosis & Therapy 12: 391 3. Wågsäter, D. et al. (2007) International J Oncol. 31: 97 4. Nagelkerke, F. et al. (2008) Clinical Cancer Research 14: 2276 Technical ProtocolsIHC FFPE N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
