Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse CXCL5 (Accession # P50228) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828473 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 1 µg/ml. A suitable detection antibody is PN:L140 at a concentration of approximately 400 ng/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 1 ng/well. A suggested standard for this assay is PN:L123. Western Blotting: To detect Mouse CXCL5 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL5 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L123. Other Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse CXCL5 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CXCL5 (at 0.2 ng/ml) is 0.5 - 2.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse CXCL5 (Clone 61905) recognizes an epitope on Mouse CXCL5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen Distribution Endothelial cells, fibroblasts, monocytes, macrophages, platelets Background CXCL5 is an inflammatory chemokine that belongs to the CXC chemokine family1 that has a role in the development of cardiovascular diseases.2 CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils.1 It is expressed by epithelial cells within colorectal mucosa is a chemoattractant for neutrophils and has been implicated in Crohn's disease and ulcerative colitis.3 CXCL5 is important in growth and development of colorectal cancer, implicating a future role in both cancer therapy and diagnosis.4 Antigen DetailsProtein PubMed References & Citations1. Rajashekhar, G. et al. (2007) Physiol Genomics 31: 104 2. Mahsa, M. et al. (2008) Molec Diagnosis & Therapy 12: 391 3. Wågsäter, D. et al. (2007) International J Oncol. 31: 97 4. Nagelkerke, F. et al. (2008) Clinical Cancer Research 14: 2276 Technical Protocols  |
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