Anti-Rat CXCL5 (Clone 135803)
Anti-Rat CXCL5 (Clone 135803)
Product No.: L217
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Clone 135803 Target CXCL5 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names AMCF-II, Epithelial-Derived Neutrophil-Activating Peptide 78 (ENA-78), GCP-2, Scyb5, Scyb6, Lipopolysaccharide-Induced CXC Chemokine (LIX) Isotype IgG1 Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Mouse Immunogen Purified Recombinant Rat CXCL5 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831147 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml. A suitable detection antibody is PN:L231 at a concentration of approximately 100 ng/ml. A suggested standard for this assay is PN:L198. Western Blotting: To detect Rat CXCL5 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat CXCL5 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L198. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat LIX bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat LIX (at 0.2 ng/ml) is 1-5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Rat CXCL5 (Clone 135803) recognizes an epitope on Rat CXCL5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background CXCL5 is an inflammatory chemokine that belongs to the CXC chemokine family1 that has a role in the development of cardiovascular diseases.2 CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils.1 It is expressed by epithelial cells within colorectal mucosa is a chemoattractant for neutrophils and has been implicated in Crohn's disease and ulcerative colitis.3 CXCL5 is important in growth and development of colorectal cancer, implicating a future role in both cancer therapy and diagnosis.4 Antigen DetailsPubMed References & Citations1. Rajashekhar, G. et al. (2007) Physiol Genomics 31: 104 2. Mahsa, M. et al. (2008) Molec Diagnosis & Therapy 12: 391 3. Wågsäter, D. et al. (2007) International J Oncol. 31: 97 4. Nagelkerke, F. et al. (2008) Clinical Cancer Research 14: 2276 Technical Protocols N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
