Recombinant Mouse IL-13

Interleukin 13 (IL-13) is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells¹, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα), which is also a component of the IL-4 receptor, and at least one of two known IL-13-specific binding chains.¹ The functions of IL-13 overlap considerably with those of IL-4. Although IL-13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.³ The ED₅₀ for this effect is 0.75‑3 ng/mL.

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Ser26

Lyophilized

The predicted molecular weight of Recombinant Mouse IL-13 is Mr 11.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 9 kDa.

11.5

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Wynn, TA. et al. (2003) Annu Rev Immunol. 21: 425
2. Citrin, DE. et al. (2016) Sci Rep. 6:39714.
3. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.