Anti-Mouse IL-13 (Clone 38213)

Pricing & Details

Product No.I-665
Clone
38213
Protein
IL-13
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
ALRH, BHR1, MGC116786, P600, NC30, Interleukin-13
Isotype
Rat
IgG2a
Applications
ELISA Cap
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-665-25 µg
25 µg
$225.00
In stock
Max:
Min: 1
Step: 1
I-665-100 µg
100 µg
$425.00
In stock
Max:
Min: 1
Step: 1
I-665-500 µg
500 µg
$925.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse IL-13 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml. A suitable detection antibody is PN:I-349 at a concentration of approximately 0.1-0.4 µg/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 4 ng/well. A suggested standard for this assay is PN:I-278.
Western Blotting: To detect Mouse IL-13 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-13 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-278.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse IL-13 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IL-13 (at 10 ng/ml) is 0.3 - 0.8 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Interleukin 13 (IL-13) (Clone 38213) recognizes an epitope on Mouse IL-13. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Interleukin 13 (IL-13) is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells1, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα), which is also a component of the IL-4 receptor, and at least one of two known IL-13-specific binding chains.1 The functions of IL-13 overlap considerably with those of IL-4. Although IL-13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.

Antigen Details

Protein
PubMed

References & Citations

1. Wynn, TA. et al. (2003) Annu Rev Immunol. 21: 425
2. Citrin, DE. et al. (2016) Sci Rep. 6:39714.
Elisa Sandwich Protocol
General Western Blot Protocol

Formats Available

Prod No.
Description
I-665
Products are for research use only. Not for use in diagnostic or therapeutic procedures.