Anti-Mouse IL-13 (Clone 38213)

Product No.: I-665


Clone 38213 Target IL-13 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names ALRH, BHR1, MGC116786, P600, NC30, Interleukin-13 Isotype IgG2a Applications ELISA Cap , N , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse IL-13 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830786 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml. A suitable detection antibody is PN:I-349 at a concentration of approximately 0.1-0.4 µg/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 4 ng/well. A suggested standard for this assay is PN:I-278. Western Blotting: To detect Mouse IL-13 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-13 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-278. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse IL-13 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse IL-13 (at 10 ng/ml) is 0.3 - 0.8 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Rat Anti-Mouse Interleukin 13 (IL-13) (Clone 38213) recognizes an epitope on Mouse IL-13. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Interleukin 13 (IL-13) is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells¹, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα), which is also a component of the IL-4 receptor, and at least one of two known IL-13-specific binding chains.¹ The functions of IL-13 overlap considerably with those of IL-4. Although IL-13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction. PubMed IL-13 Research Area Other Molecules References & Citations 1. Wynn, TA. et al. (2003) Annu Rev Immunol. 21: 425 2. Citrin, DE. et al. (2016) Sci Rep. 6:39714. View product citations for antibody I-665 on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse IL-13 (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830786

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml. A suitable detection antibody is PN:I-349 at a concentration of approximately 0.1-0.4 µg/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 4 ng/well. A suggested standard for this assay is PN:I-278.
Western Blotting: To detect Mouse IL-13 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-13 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-278.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Mouse IL-13 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse IL-13 (at 10 ng/ml) is 0.3 - 0.8 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Interleukin 13 (IL-13) (Clone 38213) recognizes an epitope on Mouse IL-13. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Interleukin 13 (IL-13) is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells¹, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα), which is also a component of the IL-4 receptor, and at least one of two known IL-13-specific binding chains.¹ The functions of IL-13 overlap considerably with those of IL-4. Although IL-13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.

PubMed

IL-13

Research Area

Other Molecules

References & Citations

1. Wynn, TA. et al. (2003) Annu Rev Immunol. 21: 425
2. Citrin, DE. et al. (2016) Sci Rep. 6:39714.

View product citations for antibody I-665 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
I-634	Anti-Human IL-13 (Clone 32116)
I-665	Anti-Mouse IL-13 (Clone 38213)
I-854	Anti-Rat IL-13
I-874	Anti-Rat IL-13 (Clone 296202)
I-348	Anti-Mouse IL-13
I-278	Recombinant Mouse IL-13
I-220	Recombinant Rat IL-13
I-269	Anti-Human IL-13 – Biotin
I-349	Anti-Mouse IL-13 – Biotin
I-864	Anti-Rat IL-13 – Biotin
I-195	Recombinant Human IL-13

Formats Available

Prod No.	Description
I-665	Anti-Mouse IL-13 (Clone 38213)

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Anti-Mouse IL-13 (Clone 38213)