Anti-Human IL-13 (Clone 32116)
Anti-Human IL-13 (Clone 32116)
Product No.: I-634
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Clone 32116 Target IL-13 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-13, ALRH, BHR1, MGC116786, P600, NC30 Isotype IgG1 Applications CyTOF® , ELISA Cap , FC , N |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human IL-13 (Accession # P35225) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830756 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 - 8 µg/ml. A suitable detection antibody is PN:I-269 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-195. Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human IL-13 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-13 (at 10 ng/ml) is 1-3 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Interleukin 13 (IL-13) (Clone 32116) recognizes an epitope on Human IL-13. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Interleukin 13 (IL-13) is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells1, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα), which is also a component of the IL-4 receptor, and at least one of two known IL-13-specific binding chains.1 The functions of IL-13 overlap considerably with those of IL-4. Although IL-13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction. Antigen DetailsPubMed Research Area Other Molecules References & Citations1. Wynn, TA. et al. (2003) Annu Rev Immunol. 21: 425 2. Citrin, DE. et al. (2016) Sci Rep. 6:39714. Technical ProtocolsCyTOF®   N |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
