Anti-Rat IL-13 (Clone 296202)
Anti-Rat IL-13 (Clone 296202)
Product No.: I-874
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Clone 296202 Target IL-13 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-13 Isotype IgG2b Applications N , WB |
Data
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Mouse Immunogen Purified Recombinant Rat IL-13 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830960 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat IL-13 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as and a chromogenic substrate such as PN:T343. The detection limit for Rat IL-13 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-220. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat IL-13 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat IL-13 (at 15 ng/ml) is 1-4 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Rat Interleukin 13 (IL-13) (Clone 296202) recognizes an epitope on Rat IL-13. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org Research Area Other Molecules References & Citations1. Wynn, TA. et al. (2003) Annu Rev Immunol. 21: 425 2. Citrin, DE. et al. (2016) Sci Rep. 6:39714. Technical ProtocolsN  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
