Recombinant Mouse L-Selectin

Recombinant Mouse L-Selectin

Product No.: L199

[product_table name="All Top" skus="L199"]

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Alternate Names
CD62L, SELL, LAM-1, LECAM1, LNHR, LSEL, LYAM1, Leu-8, Lyam-1, PLNHR, TQ1, hLHRc, MEL-14 Antigen, DREG
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Mouse

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Product Size
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Background

L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain1 found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.2

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse L-Selectin was determined by the ability of the immobilized protein to support the adhesion of LS180 cells. When 1 x 10<sup>4</sup> cells/well are added to L-Selectin coated plates approximately 90 - 100% will adhere for 1 hour incubation at RT.
Fusion Protein Tag
Fc Fusion Protein
Protein Accession No.
Amino Acid Sequence
wt yhysekpmnw enarkfckqn ytdlvaiqnk reieylentl pkspyyywig irkigkmwtw vgtnktltke aenwgagepn nkkskedcve iyikrerdsg kwnddachkr kaalcytasc qpgscngrge cvetinnhtc icdagyygpq cqyvvqcepl eapelgtmdc ihplgnfsfq skcafncseg rellgtaetq cgasgnwssp epicqvvqce pleapelgtm dcihplgnfs fqskcafncs egrellgtae tqcgasgnws spepicqetn rsfskikegd yniegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgkh hhhhh
N-terminal Sequence Analysis
Trp39
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse L-Selectin is Mr 60.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 150 kDa.
Predicted Molecular Mass
60.5
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Levy, K. et al. (2005) J. Cell Mol. Med. 9: 255
2. Spangrude, GJ. et al. (2004) Blood 103: 2990
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.