Recombinant Mouse L-Selectin

L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain¹ found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.²

The biological activity of Mouse L-Selectin was determined by the ability of the immobilized protein to support the adhesion of LS180 cells. When 1 x 10⁴ cells/well are added to L-Selectin coated plates approximately 90 - 100% will adhere for 1 hour incubation at RT.

Fc Fusion Protein

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Trp39

Lyophilized

The predicted molecular weight of Recombinant Mouse L-Selectin is Mr 60.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 150 kDa.

60.5

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Levy, K. et al. (2005) J. Cell Mol. Med. 9: 255
2. Spangrude, GJ. et al. (2004) Blood 103: 2990