Anti-Mouse L-Selectin (Clone 95205)

Anti-Mouse L-Selectin (Clone 95205)

Product No.: L218

[product_table name="All Top" skus="L218"]

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Clone
95205
Target
L-Selectin
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
SELL, CD62L, LAM-1, LECAM1, LNHR, LSEL, LYAM1, Leu-8, Lyam-1, PLNHR, TQ1, hLHRc
Isotype
IgG1
Applications
FC
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse L-Selectin Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing L-Selectin. A suggested method would be to stain cells expressing L-Selectin with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:R485.
Western Blotting: To detect Mouse L-Selectin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse L-Selectin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse L-Selectin (Clone 95205) recognizes an epitope on Mouse L-Selectin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhL-Selectin, rmP-Selectin or rmE-Selectin.
Background
L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain1 found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.2

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Levy, K. et al.(2005) J. Cell Mol. Med. 9: 255
2. Spangrude, GJ. et al.(2004) Blood 103: 2990
Flow Cytometry
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
L218
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.