Anti-Mouse L-Selectin (Clone 95205)
Anti-Mouse L-Selectin (Clone 95205)
Product No.: L218
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Clone 95205 Target L-Selectin Formats AvailableView All Product Type Monoclonal Antibody Alternate Names SELL, CD62L, LAM-1, LECAM1, LNHR, LSEL, LYAM1, Leu-8, Lyam-1, PLNHR, TQ1, hLHRc Isotype IgG1 Applications FC , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen NS0-Derived Recombinant Mouse L-Selectin Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831148 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing L-Selectin. A suggested method would be to stain cells expressing L-Selectin with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:R485. Western Blotting: To detect Mouse L-Selectin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse L-Selectin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse L-Selectin (Clone 95205) recognizes an epitope on Mouse L-Selectin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with
rhL-Selectin, rmP-Selectin or rmE-Selectin. Background L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain1 found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.2 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Levy, K. et al.(2005) J. Cell Mol. Med. 9: 255 2. Spangrude, GJ. et al.(2004) Blood 103: 2990 Technical Protocols  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
