Anti-Mouse L-Selectin (Clone 95205)

Pricing & Details


Product No.L218 Clone 95205 Protein L-Selectin Formats AvailableView All Product Type Monoclonal Antibody Alternate Names SELL, CD62L, LAM-1, LECAM1, LNHR, LSEL, LYAM1, Leu-8, Lyam-1, PLNHR, TQ1, hLHRc Isotype Rat IgG1 Applications FC , WB Submit A Review	Printable Datasheet Printable SDS

Prod No.	Size	Price	Avail.	Qty	Add to cart
L218-25 µg	25 µg	$170.00	In stock	Max: Min: 1 Step: 1	Add to Cart
L218-500 µg	500 µg	$525.00	In stock	Max: Min: 1 Step: 1	Add to Cart


Antibody Details Product Details Reactivity Species Mouse Host Species Rat Immunogen NS0-Derived Recombinant Mouse L-Selectin Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831148 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing L-Selectin. A suggested method would be to stain cells expressing L-Selectin with 0.25 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of ≤200 µl followed by PN:R485. Western Blotting: To detect Mouse L-Selectin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse L-Selectin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Rat Anti-Mouse L-Selectin (Clone 95205) recognizes an epitope on Mouse L-Selectin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhL-Selectin, rmP-Selectin or rmE-Selectin. Background L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain¹ found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.² Antigen Details Protein L-Selectin PubMed L-Selectin NCBI Gene Bank ID 20343 UniProt.org Information on Uniprot.org References & Citations 1. Levy, K. et al.(2005) J. Cell Mol. Med. 9: 255 2. Spangrude, GJ. et al.(2004) Blood 103: 2990 Technical Protocols

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

NS0-Derived Recombinant Mouse L-Selectin Extracellular Domain

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831148

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing L-Selectin. A suggested method would be to stain cells expressing L-Selectin with 0.25 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of ≤200 µl followed by PN:R485.
Western Blotting: To detect Mouse L-Selectin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse L-Selectin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse L-Selectin (Clone 95205) recognizes an epitope on Mouse L-Selectin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhL-Selectin, rmP-Selectin or rmE-Selectin.

Background

L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain¹ found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.²

Antigen Details

Protein

L-Selectin

PubMed

L-Selectin

NCBI Gene Bank ID

20343

UniProt.org

Information on Uniprot.org

References & Citations

1. Levy, K. et al.(2005) J. Cell Mol. Med. 9: 255
2. Spangrude, GJ. et al.(2004) Blood 103: 2990

Prod No.	Description
C2275	Anti-Human CD62L – PerCP
C2271	Anti-Human CD62L – FITC
C2140	Anti-Human CD62L – Purified
C2276	Anti-Human CD62L – DyLight® 488
C2277	Anti-Human CD62L – DyLight® 550
C2278	Anti-Human CD62L – DyLight® 594
C2279	Anti-Human CD62L – DyLight® 650
C2274	Anti-Human CD62L – PE
C2270	Anti-Human CD62L – Purified in vivo GOLD™ Functional Grade
C2273	Anti-Human CD62L – APC
L218	Anti-Mouse L-Selectin (Clone 95205)
L222	Anti-Mouse L-Selectin
L224	Anti-Human L-Selectin
C2272	Anti-Human CD62L – Biotin
L203	Recombinant Human L-Selectin
L233	Anti-Human L-Selectin – Biotin
L238	Recombinant Human L-Selectin (Leukocyte Selectin)

Formats Available

Prod No.	Description
L218	Anti-Mouse L-Selectin (Clone 95205)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Anti-Mouse L-Selectin (Clone 95205)