Anti-Human CD62L – Purified in vivo GOLD™ Functional Grade

Product No.: C2270

[product_table name="All Top" skus="C2270"]

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Clone
DREG56
Target
CD62L
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TQ1, LAM1, LEU8, LNHR, LSEL, CD62L, LYAM1, PLNHR, LECAM1, L-selectin
Isotype
IgG1
Applications
B
,
FC
,
IF
,
IHC FF
,
in vivo
,
PhenoCycler®
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Concentrated supernatant from PMA-activated human peripheral blood leukocytes
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this DREG56 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this DREG56 antibody for use in western blotting is 1-10 μg/ml.
Additional Applications Reported In Literature ?
IHC FF
IF
B
Additional Reported Applications For Relevant Conjugates ?
PhenoCycler-Fusion (CODEX)®
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone DREG56 recognizes an epitope on human CD62L.
Background
CD62L antibody, clone DREG56, recognizes CD62L, also known as L-selectin or LECAM-1, a 74-95 kDa type I transmembrane glycoprotein and cell adhesion molecule expressed on most circulating leukocytes1. Ligands for CD62L include CD34, GlyCAM-1, and MAdCAM-1, which require sialyation, fucosylation, and carbohydrate sulfation for recognition2,3. Ligand binding mediates tethering and rolling of leukocytes, facilitating entry into secondary lymphoid organs, including lymph nodes and Peyer’s patches, via high endothelial venules (HEVs)1. CD62L is also expressed on human embryo trophoblasts and has been proposed to play a role in human embryo implantation4.
Antigen Distribution
CD62L is expressed on most circulating leukocytes, including neutrophils, monocytes, T, B and NK cells, and human embryo trophoblasts.

Antigen Details

Ligand/Receptor
CD34, GlyCAM, MAdCAM-1
Function
Leukocyte homing, leukocyte tethering, rolling
PubMed
NCBI Gene Bank ID
Research Area
Cell Adhesion
.
Cell Biology
.
Costimulatory Molecules
.
Immunology
.
Innate Immunity

References & Citations

1. Ivetic A, et al. (2019) Front Immunol. 10:1068
2. Varki A (1994) Proc Natl Acad Sci USA. 91(16):7390-7397
3. Rosen SD. (1999) Am J Pathol. 155(4):1013-1020
4. Genbacev OD, et al. (1003) Science. 299(5605):405-8
B
Flow Cytometry
IF
IHC FF
in vivo Protocol
PhenoCycler®
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.