Anti-Human CD62L – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Human CD62L – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C512

[product_table name="All Top" skus="C2140"]

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Clone
DREG56
Target
CD62L
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TQ1, LAM1, LEU8, LNHR, LSEL, CD62L, LYAM1, PLNHR, LECAM1
Isotype
IgG1
Applications
IHC FF
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone DREG56 has been validated for use on CODEX® using human tonsil Fresh Frozen (FF) tissue.
Tissue Screened
Tonsil
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Human
Host Species
Mouse
Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD62L (Clone DREG56) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893569
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone DREG56 recognizes an epitope on human CD62L.
Antigen Distribution
CD62L is expressed on most circulating leukocytes, including neutrophils, monocytes, T, B and NK cells, and human embryo trophoblasts.
Background
CD62L antibody, clone DREG56, recognizes CD62L, also known as L-selectin or LECAM-1, a 74-95 kDa type I transmembrane glycoprotein and cell adhesion molecule expressed on most circulating leukocytes1. Ligands for CD62L include CD34, GlyCAM-1, and MAdCAM-1, which require sialyation, fucosylation, and carbohydrate sulfation for recognition2,3. Ligand binding mediates tethering and rolling of leukocytes, facilitating entry into secondary lymphoid organs, including lymph nodes and Peyer’s patches, via high endothelial venules (HEVs)1. CD62L is also expressed on human embryo trophoblasts and has been proposed to play a role in human embryo implantation4.

Antigen Details

Protein
Ligand/Receptor
CD34, GlyCAM, MAdCAM-1
Function
Leukocyte homing, leukocyte tethering, rolling
PubMed
NCBI Gene Bank ID

References & Citations

1. Ivetic A, et al. (2019) Front Immunol. 10:1068
2. Varki A (1994) Proc Natl Acad Sci USA. 91(16):7390-7397
3. Rosen SD. (1999) Am J Pathol. 155(4):1013-1020
4. Genbacev OD, et al. (1003) Science. 299(5605):405-8
IHC
PhenoCycler®

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.