Recombinant Human L-Selectin (Leukocyte Selectin)

Recombinant Human L-Selectin (Leukocyte Selectin)

Product No.: L238

[product_table name="All Top" skus="L238"]

- -
- -
Prod. No.L238
Expression Host
CHO Cells

- -
- -
Select Product Size
- -
- -

Background

Human LSelectin (Leukocyte Selectin, LAM1,LECAM1,LECCAM1,TQ1, Leu8,MEL14 antigen, DREG, lymph node homing receptor, CD62L), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes.

Protein Details

Purity
>97% by SDS Page and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
Recombinant Human L-Selectin was measured by its ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells. When 5 x 104 cells/well are added to human LSelectin coated plates (10 μg/mL with 100 μL/well), >50% will adhere after 1 hour incubation at 37 °C.
Protein Accession No.
Amino Acid Sequence
wtyhysekpm nwqrarrfcr dnytdlvaiq nkaeieylek tlpfsrsyyw igirkiggiw twvgtnkslt eeaenwgdge pnnkknkedc veiyikrnkd agkwnddach klkaalcyta scqpwscsgh gecveiinny tcncdvgyyg pqcqfviqce pleapelgtm dcthplgnfs fssqcafscs egtnltgiee ttcgpfgnws speptcqviq ceplsapdlg imncshplas fsftsactfi csegteligk kkticessgi wsnpspicqk ldksfsmike gdyn
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, CD62L is Mr 33.1 kDa.
Predicted Molecular Mass
33.1
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives. 1% sorbitol was added as a stabilizer.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 105 continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.
Elisa Sandwich Protocol
Flow Cytometry
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.