Recombinant Mouse Resistin

Resistin, also known as ADSF, RETN, CEBPE, is a cysteine-rich protein that belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. Resistin is secreted by adipocytes, pituitary and hypothalamus with potential roles in insulin resistance and adipocyte differentiation (1-2). In addition, Resistin functions as a regulator of glucose homeostasis and a physiologic antagonist to hepatic insulin action (3). Resistin may be an important mediator of obesity-induced insulin resistance and may represent a novel link between metabolic signals, inflammation, and atherosclerosis (4-5).

The biological activity of Mouse Resistin was determined by its ability to inhibit mouse 3T3-L1 cell adipocyte differentiation. The expected ED₅₀ for this effect is typically 5-25 µg/ml.

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Ser21

Lyophilized

Recombinant mouse Resistin contains a mixture of monomeric and disulfide-linked homodimeric proteins. The predicted molecular weight of Recombinant Mouse Resistin monomer is Mr 10 kDa.

10

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable ≥ twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution and addition of a carrier protein, this protein may be stored under sterile conditions at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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1. Lazar, MA. et al. (2001) J. Biol. Chem. 276:25970
2. Lazar, MA. et al. (2002) J. Biol. Chem. 277:19754
3. Wolf, G. et al. (2004) Nutr. Rev. 62:389
4. Chen, R. et al. (2004) Eur. J. Endocrinol. 150:885
5. Rader, DJ. et al. (2005) Circulation 111:9