Anti-Mouse Resistin – Biotin

Anti-Mouse Resistin – Biotin

Product No.: R1159

[product_table name="All Top" skus="R1159"]

- -
- -
Target
Resistin
Product Type
Polyclonal Antibody
Alternate Names
Adipocyte-Specific Secretory Factor (ADSF), Inflammatory Zone 3 (FIZZ3), MGC126603, MGC126609, RETN1, RSTN, CEBPE Regulated Myeloid-Specific Secreted Cysteine-Rich Protein Precursor 1 (XCP1)
Applications
ELISA Det
,
WB

- -
- -
Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse Resistin (Accession # Q99P87)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse Resistin this biotin conjugate can be used at a concentration of 0.1 µg/ml.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1 - 0.4 µg/ml when used in conjunction a capture antibody and an optimal second step reagent such as PN:A106 for the detection of Mouse Resistin.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse Resistin recognizes Mouse Resistin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Resistin (RETN) is a peptide hormone secreted by adipocytes 1 with potential roles in insulin resistance and adipocyte differentiation.2 Resistin functions as a regulator of glucose homeostasis and a physiologic antagonist to hepatic insulin action.3 Resistin may be an important candidate mediator of obesity-induced insulin resistance.4 It may also represent a novel link between metabolic signals, inflammation, and atherosclerosis.5

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Rader, DJ. et al. (2005) Circulation 111: 932
2. Chen, R. et al. (2004) Eur J Endocrinol. 150: 885
3. Wolf, G. et al. (2004) Nutr Rev. 62: 389
4. Lazar, MA. et al. (2002) J Biol Chem. 277: 19754
5. Lazar, MA. et al. (2001) J Biol Chem. 276: 25970
ELISA Det
General Western Blot Protocol
- -
- -
Products are for research use only. Not for use in diagnostic or therapeutic procedures.