Recombinant Rat Jagged-1

Jagged 1 (JAG1) is a cell surface protein that functions in an embryologically important signaling pathway, known as the Notch signaling pathway, which is a highly conserved cell signaling system.¹ JAG1 plays an essential role in tissue formation during embryonic development of primitive organisms.² JAG1 is a strong candidate gene for Alagille syndrome (AGS)³ which is an autosomal dominant disorder with developmental abnormalities affecting the liver, heart, eyes, vertebrae, and craniofacial region.⁴

Fc Fusion Protein

sgqfeleils mqnvngelqn gnccgnpgdr kctrdecdty fkvclkeyqs rvtaggpcsf gsgstpvigg ntfnlkasrg ndrnrivlpf sfawprsytl lveawdssnd tiqpdsiiek ashsgminps rqwqtlkqnt giahfeyqir vtcddhyygf gcnkfcrprd dffghyacdq ngnktcmegw mgpecnkaic rqgcspkhgs cklpgdcrcq ygwqglycdk ciphpgcvhg tcnepwqclc etnwggqlcd kdlnycgthq pclnrgtcsn tgpdkyqcsc pegysgpnce iaehaclsdp chnrgscket ssgfececsp gwtgptcstn iddcspnncs hggtcqdlvn gfkcvcppqw tgktcqldan eceakpcvna rscknliasy ycdclpgwmg qncdinindc lgqcqndasc rdlvngyrci cppgyagdhc erdidecasn pclngghcqn einrfqclcp tgfsgnlcql didycepnpc qngaqcynra sdyfckcped yegkncshlk dhcrttpcev idsctvamas ndtpegvryi ssnvcgphgk cksesggkft cdcnkgftgt ychenindce gnpctnggtc idgvnsykci csdgwegahc ennindcsqn pchyggtcrd lvndfycdck ngwkgktchs rdsqcdeatc nnggtcydev dtfkcmcpgg wegttcniar nssclpnpch nggtcvvngd sftcvckegw egpictqntn dcsphpcyns gtcvdgdnwy rcecapgfag pdcrininec qsspcafgat cvdeingyqc icppghsgak chevsgrsci tmgrvildga kwdddcntcq clngrvacsk vwcgprpcrl hkghgecpng qscipvlddq cfvrpctgag ecrssslqpv ktkctsdsyy qdncanitft fnkemmspgl ttehicselr nlnilknvsa eysiyiacep slsanneihv aisaedirdd gnpvkeitdk iidlvskrdg nssliaavae vrvqrrplkn rtdfiegrmd pkscdkthtc ppcpapellg gpsvflfppk pkdtlmisrt pevtcvvvdv shedpevkfn wyvdgvevhn aktkpreeqy nstyrvvsvl tvlhqdwlng keykckvsnk alpapiekti skakgqprep qvytlppsrd eltknqvslt clvkgfypsd iavewesngq pennykttpp vldsdgsffl yskltvdksr wqqgnvfscs vmhealhnhy tqkslslspg khhhhhh (Gly57-Arg59 deleted, Asp63Thr, Arg64Leu, and Val-Arg-Pro-Tyr inserted before Lys69)

Ser32

Lyophilized

The predicted molecular weight of Recombinant Rat Jagged 1 is Mr 141.7 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 180 kDa.

141.7

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Spinner, NB. et al. (2001) Semin Liver Dis. 21: 525
2. Bhatia, M. et al. (2000) J Exp Med. 192: 1365
3. Chandrasekharappa, SC. et al. (1997) Genomics 43: 376
4. DeVirgilis, S. et al. (1999) Hum Mutat. 14: 394