Antibody DetailsProduct DetailsReactivity Species Rat Host Species Mouse Immunogen NS0-Derived Recombinant Rat Jagged 1 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831066 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat Jagged-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat Jagged-1 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Rat Jagged-1 (Clone 72017) recognizes an epitope on Rat Jagged-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody showed no cross-reactivity with rhJagged 1. Background Jagged 1 (JAG1) is a cell surface protein that functions in an embryologically important signaling pathway, known as the Notch signaling pathway, which is a highly conserved cell signaling system.1 JAG1 plays an essential role in tissue formation during embryonic development of primitive organisms.2 JAG1 is a strong candidate gene for Alagille syndrome (AGS)3 which is an autosomal dominant disorder with developmental abnormalities affecting the liver, heart, eyes, vertebrae, and craniofacial region.4 Antigen DetailsProtein PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Spinner, NB. et al. (2001) Semin Liver Dis. 21: 525 2. Bhatia, M. et al. (2000) J Exp Med. 192: 1365 3. Chandrasekharappa, SC. et al. (1997) Genomics 43: 376 4. DeVirgilis, S. et al. (1999) Hum Mutat. 14: 394 Technical Protocols |
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