Anti-Rat Jagged-1
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Goat Immunogen NS0 - Derived Recombinant Rat Jagged 1 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831067 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:G505. The detection sensitivity of this indirect ELISA for Rat Jagged 1 is approximately 0.16 ng/well. Western Blotting: To detect Rat Jagged 1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Rat Jagged 1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat Jagged 1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat Jagged 1 (at 200 ng/ml) is 2 - 10 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Rat Jagged 1 recognizes Rat Jagged 1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Jagged 1 (JAG1) is a cell surface protein that functions in an embryologically important signaling pathway, known as the Notch signaling pathway, which is a highly conserved cell signaling system.1 JAG1 plays an essential role in tissue formation during embryonic development of primitive organisms.2 JAG1 is a strong candidate gene for Alagille syndrome (AGS)3 which is an autosomal dominant disorder with developmental abnormalities affecting the liver, heart, eyes, vertebrae, and craniofacial region.4 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Spinner, NB. et al. (2001) Semin Liver Dis. 21: 525 2. Bhatia, M. et al. (2000) J Exp Med. 192: 1365 3. Chandrasekharappa, SC. et al. (1997) Genomics 43: 376 4. DeVirgilis, S. et al. (1999) Hum Mutat. 14: 394 Technical Protocols  N  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
