Anti-Rat Jagged-1 - Biotin - Leinco Technologies

Anti-Rat Jagged-1 – Biotin

Product No.: J110


Target Jagged-1 Product Type Polyclonal Antibody Alternate Names AGS, AHD, AWS, Cluster of Differentiation (CD339), HJ1, JAGL1, MGC104644 Applications ELISA Det , WB


Select Product Size 50 µg — $550.00	Qty Max: Min: 1 Step: 1 $550.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Rat Host Species Goat Immunogen NS0-Derived Recombinant Rat Jagged 1 Extracelluar Domain (Accession # Q63722) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831068 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat Jagged 1 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for rrJagged 1 is approximately 5 ng/lane and 50 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL. Use in combination with: Capture Reagent: Rat Jagged 1 Antibody. Standard: Recombinant Rat Jagged 1 Fc Chimera Protein, CF. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Rat Jagged 1 recognizes Rat Jagged 1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Jagged 1 (JAG1) is a cell surface protein that functions in an embryologically important signaling pathway, known as the Notch signaling pathway, which is a highly conserved cell signaling system.¹ JAG1 plays an essential role in tissue formation during embryonic development of primitive organisms.² JAG1 is a strong candidate gene for Alagille syndrome (AGS)³ which is an autosomal dominant disorder with developmental abnormalities affecting the liver, heart, eyes, vertebrae, and craniofacial region.⁴ PubMed Jagged-1 NCBI Gene Bank ID 29146 UniProt.org More Information on Uniprot.org References & Citations 1. Spinner, NB. et al. (2001) Semin Liver Dis. 21: 525 2. Bhatia, M. et al. (2000) J Exp Med. 192: 1365 3. Chandrasekharappa, SC. et al. (1997) Genomics 43: 376 4. DeVirgilis, S. et al. (1999) Hum Mutat. 14: 394 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols ELISA Det Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Rat

Host Species

Goat

Immunogen

NS0-Derived Recombinant Rat Jagged 1 Extracelluar Domain (Accession # Q63722)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831068

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Rat Jagged 1 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for rrJagged 1 is approximately 5 ng/lane and 50 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL. Use in combination with: Capture Reagent: Rat Jagged 1 Antibody. Standard: Recombinant Rat Jagged 1 Fc Chimera Protein, CF.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Rat Jagged 1 recognizes Rat Jagged 1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Jagged 1 (JAG1) is a cell surface protein that functions in an embryologically important signaling pathway, known as the Notch signaling pathway, which is a highly conserved cell signaling system.¹ JAG1 plays an essential role in tissue formation during embryonic development of primitive organisms.² JAG1 is a strong candidate gene for Alagille syndrome (AGS)³ which is an autosomal dominant disorder with developmental abnormalities affecting the liver, heart, eyes, vertebrae, and craniofacial region.⁴

PubMed

Jagged-1

NCBI Gene Bank ID

29146

UniProt.org

More Information on Uniprot.org

References & Citations

1. Spinner, NB. et al. (2001) Semin Liver Dis. 21: 525
2. Bhatia, M. et al. (2000) J Exp Med. 192: 1365
3. Chandrasekharappa, SC. et al. (1997) Genomics 43: 376
4. DeVirgilis, S. et al. (1999) Hum Mutat. 14: 394

View product citations for antibody PRODUCT_CODE on CiteAb

ELISA Det

Certificate of Analysis

Request COA

Prod No.	Description
J108	Anti-Rat Jagged-1 (Clone 72017)
J109	Anti-Rat Jagged-1
J106	Recombinant Rat Jagged-1
J110	Anti-Rat Jagged-1 – Biotin

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Rat Jagged-1 – Biotin