Streptavidin — Alexa Fluor™ 647

Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (K_d~10 ^-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.

Streptavidin-Alexa Fluor™ 647 is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA and 0.09% sodium azide as a preservative.

Keep the streptavidin-Alexa Fluor™ 647 solution undiluted at 2°–8°C. Protect from prolonged light exposure and do not freeze.

2-8°C

Flow Cytometry Staining: Use ≤ 0.125 µg of reagent per million cells in a 100 µl volume. For optimal performance, titrate the reagent for your specific application.